Recent Publications

Li, M., Mizuuchi, M., Burke, T.R., and Craigie, R. (2006) Retroviral DNA integration: reaction pathway and critical intermediates. EMBO J. 25, 1295-1304.

Bradley C.M., and Craigie R. (2005) Seeing is believing: structure of the catalytic domain of HIV-1 integrase in complex with human LEDGF/p75. Proc Natl Acad Sci U S A 102, 17543-417504.

Bradley, C.M., Ronning, D.R., Ghirlando, R., Craigie, R., and Dyda, F. (2005) Structural basis for DNA bridging by barrier-to-autointegration factor. Nature Structural and Mol. Biol. 12, 935-936.

Li , M. and Craigie, R. (2005) Processing of viral DNA ends channels the HIV-1 integration reaction to concerted integration. J. Biol. Chem. 280, 29334-29339.

Williams, K.L., Zhang, Y.J., Shkriabai, N., Karki ,R.G., Nicklaus, M.C., Kotrikadze, N., Hess, S., Le Grice, S.F.J., Craigie, R., Pathak, V.K., and Kvaratskhelia, M. (2005) Mass spectrometric analysis of the HIV-1 integrase-pyridoxal 5 '-phosphate complex reveals a new binding site for a nucleotide inhibitor. J. Biol. Chem. 280, 7949-7955.

Suzuki, Y., Yang, H.F., and Craigie, R. (2004) LAP2 alpha and BAF collaborate to organize the Moloney murine leukemia virus preintegration complex. EMBO J. 23, 4670-4678.

Shkriabai, N., Patil, S.S., Hess, S., Budihas, S.R., Craigie R, Burke, T.R., Le Grice S.F.J. and Kvaratskhelia, M. (2004) Identification of an inhibitor-binding site to HIV-1 integrase with affinity acetylation and mass spectrometry. Proc. Natl. Acad. Sci. USA 101, 6894-6899.

Bradley C, Craigie R (2003) MoMLV reverse transcriptase regulates its own expression
Cell 115, 250-251.

Craigie, R. (2002) Retroviral DNA integration. In Mobile DNA II. Craig, N.L., Craigie, R., Gellert, M., and Lambowitz, A.M. ed. pp 613-630.

Suzuki, Y. and Craigie, R. (2002). Regulatory mechanisms by which BAF blocks autointegration and stimulates intermolecular integration of Molony murine leukemia virus preintegration complexes. J. Virol. 76, 12376-12380.

Segura-Totten M, Kowalski AK, Craigie R, et al. (2002) Barrier-to-autointegration factor: major roles in chromatin decondensation and nuclear assembly. J. Cell Biol. 158, 475-485.

Wang, J-Y., Ling, H., Yang, W., and Craigie, R. (2001) Structure of a two-domain fragment of HIV-1 integrase: implications for domain organization in the intact protein. EMBO J. 20, 7333-7343.

Craigie, R. (2001) HIV integrase: a brief overview from chemistry to therapeutics. J. Biol. Chem. 276, 23213-23216.

Cai, M., Huang, Y., Ghirlando, R., Wilson, K.L., Craigie, R., and Clore G.M. (2001) Solution structure of the constant region of nuclear envelope protein LAP2 reveals two LEM-domain structures: one binds BAF and the other binds DNA. EMBO J. 20, 4399-4407.

Shumaker, D.K., Lee, K.K., Tanhehoo, Y.C., Craigie, R, and Wilson, K.L. (2001) LAP2 binds to BAF*DNA complexes: requirement for the LEM domain and modulation by variable regions. EMBO J. 20. 4399-4407.

Umland, T. C., Wei, S. Q., Craigie, R., and Davies, D. R. (2000) Structural basis of DNA bridging by barrier-to-autointegration factor. Biochemistry 39, 9130-9138.

Zheng, R., Ghirlando, R., Lee, M.S., Mizuuchi, K., Krause, M., and Craigie, R. (2000) Barrier-to-autointegration factor (BAF) bridges DNA in a discrete, higher-order nucleoprotein complex. Proc. Natl. Acad. Sci. USA 97, 8997-9002

Goldur, Y., Craigie, R., Cohen, G.H., Fujiwara, T., Yoshinaga, T., Fujishita, T., Sugimoto, H., Endo, T., Murai, H., and Davies, D.R. (1999) Structure of the HIV-1 integrase catalytic domain complexed with an inhibitor: a platform for antiviral drug design. Proc. Natl. Acad. Sci. 96, 13040-13043.

Esposito, D. and Craigie, R. (1999) HIV integrase structure and function. Advances in Virus Research 52, 319-333

Cai, M., Huang, Y., Caffrey, M., Zheng, R., Craigie, R., Clore, G.M., and Gronenborn, A.M. (1998) Solution structure of the His12-Cys mutant of the N-terminal zinc binding domain of HIV-1 integrase complexed to cadmium. Protein Science 7, 2669-2674.

Cai, M., Huang, Y., Sakaguchi, G., Clore, G.M., Gronenborn, A., and Craigie, R. (1998) An efficient and cost-effective isotope labeling protocol for proteins expressed in Escheria coli. Journal of Biomolecular NMR 11, 97-102.

Cai, M., Huang, Y., Zheng, R., Wei, S-Q., Ghirlando, R., Lee, M.S., Craigie, R., Gronenborn, A.M., and Clore, G.M. (1998) Solution structure of the cellular factor BAF responsible for protecting retroviral DNA from autointegration. Nature Structural Biology 5, 903-909.

Esposito, D., and Craigie, R. (1998) Sequence specificity of viral end DNA binding by HIV-1 integrase reveals critical regions for protein/DNA interaction. EMBO. J. 17, 5832-5843.

Goldgur, Y., Dyda, F,, Hickman , A.B., Jenkins, T.M., Craigie, R., and Davies, D.R. (1998) Three new structures of the core domain of HIV-1 integrase: An active site that binds magnesium. Proc. Natl. acad. Sci. USA 95, 9150-9154.

Lee, M.S., and Craigie, R. (1998) A previously unidentified host protein protects retroviral DNA from autointegration. Proc. Natl. Acad. Sci. USA 95, 1528-1533.

Wei, S-Q, Mizuuchi, K., and Craigie, R. (1998) Footprints on the viral DNA ends in Moloney murine leukemia virus preintegration complexes reflect a specific association with integrase. Proc. Natl. Acad. Sci. USA 95, 10535-10540.

Cai, M., Zheng, R., Caffrey, M., Craigie, R., Clore, G.M., and Gronenborn, A.M. (1997) Solution structure of the N-terminal zinc binding domain of HIV-1 integrase. Nature Structural Biology 4, 567-577.

Jenkins, T.M., Esposito, D., Engelman, A., and Craigie, R. (1997) Critical contacts between HIV-1 integrase and viral DNA identified by structure-based analysis and photo-crosslinking. EMBO J. 16, 6849-6859

Wei, S-Q., Mizuuchi, K., and Craigie, R. (1997) A large nucleoprotein assembly at the ends of the viral DNA mediates retroviral DNA integration. EMBO J. 16, 7511-7520.

Hickman, A.B., Dyda, F., and Craigie, R. (1997) Heterogeneity in recombinant HIV-1 integrase corrected by site-directed mutagenesis: the identification and elinination of a protease cleavage site. Protein Engineering 10, 601-606

Craigie, R. (1996) Quality control in Mu DNA transposition. Cell 66, 137-140.

Jenkins, T.M., Engelman, A., Ghirlando, R., and Craigie, R. (1996) A soluble active mutant of HIV-1 integrase. J. Biol. Chem. 271, 7712-7718.

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Last updated 15th May, 2006